How to restore the clumping of nanocellulose to gel after freezing

Freezing nanocellulose (such as cellulose nanofibers or cellulose nanocrystals ) usually agglomerate or form a condensed state, which may be caused by moisture crystallization and phase separation during the freezing process. To restore to gel state, try the following:

Gentle stirring: The frozen nanocellulose sample is slowly thawed to room temperature and then gently stirred using a magnetic stirrer or other stirring device. Stirring helps to break the agglomerates and redisperse the cellulose nanostructures.

Sonication: If the stirring is not enough to fully restore the gel state, ultrasonication can be used. Ultrasonic waves can effectively disperse nanocellulose and improve uniformity, but pay attention to controlling the processing time and power to avoid destroying the nanocellulose structure.

High pressure homogenization : For more stubborn agglomeration, high pressure homogenization technology can be used. This method helps re-form the uniform gel by breaking the particles through a narrow nozzle at high pressure.

Add an appropriate amount of water or solution: During the thawing and stirring process, if the moisture content of nanocellulose is insufficient, add water or the original solution in moderation to help restore the gel state.

Temperature regulation: Some nanocellulose gels are temperature sensitive, and proper increase or decrease of temperature may help them return to their gel state.

It should be noted that different types of nanocellulose responses to these methods may vary, so some experiments may be required to determine the optimal recovery method.